After 72 h for paclitaxel and doxorubicin, and seven days for tamoxifen and fulvestrant, to assess the proliferative effect of estrogen, cells were cultured in 24 well plates, in phenol red free DMEM F12 medium 11039021, Gibco supplemented with 5 v v charcoal stripped FBS csFBS, 12676029, Gibco, for five days, with or without 10 nM ОІ estradiol E2758, Sigma Aldrich
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